Synthesis and characterization of novel phenylindoles as potential probes for imaging of ƒÀ-amyloid plaques in the brain.
We synthesized a novel series of phenylindole (PI) derivatives and evaluated their biological activities as probes for imaging AƒÀ plaques in vivo. The affinity for AƒÀ plaques was assessed by an in vitro-binding assay using pre-formed synthetic AƒÀ aggregates. 2-phenyl-1H-indole (2-PI) derivatives showed high affinity for AƒÀ42 aggregates with Ki values ranging from 4 to 32 nM. 2-PI derivatives clearly stained AƒÀ plaques in an animal model of AD. In biodistribution experiments using normal mice, 2-PI derivatives displayed sufficient uptake for imaging, ranging from 1.1% to 2.6% ID/g. Although additional modifications are necessary to improve uptake by and clearance from the brain, 2-PI derivatives may be useful as a backbone structure to develop novel AƒÀ imaging agents.