An ionic polymer bead-supported lipid system
ABSTRACT
In this paper, we report on the preparation of an ionic polymer bead-supported
lipid system several hundred micrometers in diameter. The electrostatic
attractive interactions between anionic lipids and cationic polymer beads
served as a gmolecular glueh to immobilize the lipids on the beads, and
then the immobilized lipids prompted the spontaneous formation of lipid
bilayer membranes. Confocal fluorescence microscopic techniques revealed
that the lipid bilayer membranes were located along the outline of the
beads. In addition, the integrity of the lipid bilayer membranes was microscopically
confirmed by a low-molecular-weight dye (trypan blue) exclusion test.