研究内容

・ルミネセンスを利用する生体関連物質の高感度・高選択的検出法の開発と応用
・高速液体及ぴキャピラリー電気クロマトグラフィーにおける分離用担体の開発
・環境汚染物質の計測と汚染源評価に関する研究





関連文献

1. ルミネセンスを利用する生体関連物質の高感度・高選択的検出法の開発と応用

Determination of the ratio between mercaptalbumin and nonmercaptalbumin by HPLC with fluorescence probe specifically binding to albumin.
Ohyama K, Kishikawa N, Matsuo A, Imazato T, Ueki Y, Wada M, Nakashima K, Kuroda N
J Pharm Biomed Anal 88: 170-103, 2014.

Chromatographic determination of low-molecular mass unsaturated aliphatic aldehydes with peroxyoxalate chemiluminescence detection after fluorescence labeling with 4-(N,N-dimethylaminosulfonyl)-7-hydrazino-2,1,3- benzoxadiazole.
Ali MF, Kishikawa N, Ohyama K, Mohamed HA, Abdel-Wadood HM, Mahmoud AM, Imazato T, Ueki Y, Wada M, Kuroda N

Journal of Chromatography B 953-954: 147-152, 2014.

A novel lophine-based fluorescence probe and its binding to human serum albumin   
Kishikawa N, Ohyama K, Saiki A, Matsuo, A, Ali, MF, Wada M, Nakashima K, Kuroda N   
Analytica Chimica Acta, 780: 1-6, 2013

Fluorescence derivatization method for sensitive chromatographic determination of zidovudine based on the Huisgen reaction.
Maeda Y, Kishikawa N, Ohyama K, Wada M, Ikeda R, Kuroda N
Journal of Chromatography A 1355: 206-210, 2014.


2. 高速液体及びキャピラリー電気クロマトグラフィーにおける分離用担体の開発

Poly(L-lactic acid)-modified silica stationary phase for reversed-phase and hydrophilic interaction liquid chromatography.
Ohyama K, Takasago S, Kishikawa N, Kuroda N
Journal of Separation Science 38(5):720-3, 2015.

Preparation and characterization of surfactin-modified silica stationary phase for reversed-phase and hydrophilic interaction liquid chromatography.
Ohyama K, Inoue Y, Kishikawa N, Kuroda N
Journal of Chromatography A 1371: 257-260, 2014.

Characterization and Comparison of Methacrylic Acid with 2-Acrylamido-2-methyl-1-propanesulfonic Acid in the Preparation of Monolithic Column for Capillary Electrochromatography. 
Horiguchi D, Ohyama K, Masunaga T, Fujita Y, Ali, MF, Kishikawa N, Kuroda, N   
Journal of Chromatographic Science, 51(5): 425-429; 2013


3. 環境汚染物質の計測と汚染源評価に関する研究

Analytical techniques for the determination of biologically active quinones in biological and environmental samples.
Kishikawa N, Kuroda N
Journal of Pharmacytical and Biomedical Analysis 87: 261-270, 2014.

A simple and rapid chemiluminescence assay for on-site analysis of paraquat using a portable luminometer.
Kishikawa N, Higuchi S, Ohyama K, Nakashima K, Kuroda N   
Forensic Toxicology, 31(2): 301-306, 2013

Development and validation of the first assay method coupling liquid chromatography with chemiluminescence for the simultaneous determination of menadione and its thioether conjugates in rat plasma.
Elgawish MS, Shimomai C, Kishikawa N, Ohyama K, Nakashima K, Kuroda, N
Chemical Research in Toxicology, 26(9): 1409-1417, 2013